SLAH3-type anion channel expressed in poplar secretory epithelia operates in calcium kinase CPK-autonomous manner.
Identifieur interne : 001684 ( Main/Exploration ); précédent : 001683; suivant : 001685SLAH3-type anion channel expressed in poplar secretory epithelia operates in calcium kinase CPK-autonomous manner.
Auteurs : Mario Jaborsky [Allemagne] ; Tobias Maierhofer [Allemagne] ; Andrea Olbrich [Allemagne] ; María Escalante-Pérez [Allemagne] ; Heike M. Müller [Allemagne] ; Judy Simon [Allemagne] ; Elzbieta Krol [Allemagne] ; Tracey Ann Cuin [Allemagne] ; Jörg Fromm [Allemagne] ; Peter Ache [Allemagne] ; Dietmar Geiger [Allemagne] ; Rainer Hedrich [Allemagne]Source :
- The New phytologist [ 1469-8137 ] ; 2016.
Descripteurs français
- KwdFr :
- Anions (métabolisme), Arabidopsis (effets des médicaments et des substances chimiques), Arabidopsis (métabolisme), Canaux ioniques (métabolisme), Fleurs (effets des médicaments et des substances chimiques), Fleurs (métabolisme), Nectar des plantes (MeSH), Nitrates (pharmacologie), Ouverture et fermeture des portes des canaux ioniques (effets des médicaments et des substances chimiques), Populus (effets des médicaments et des substances chimiques), Populus (métabolisme), Protein kinases (métabolisme), Protéines de fusion recombinantes (métabolisme), Protéines végétales (composition chimique), Protéines végétales (métabolisme), Relation structure-activité (MeSH), Épithélium (effets des médicaments et des substances chimiques), Épithélium (métabolisme).
- MESH :
- composition chimique : Protéines végétales.
- effets des médicaments et des substances chimiques : Arabidopsis, Fleurs, Ouverture et fermeture des portes des canaux ioniques, Populus, Épithélium.
- métabolisme : Anions, Arabidopsis, Canaux ioniques, Fleurs, Populus, Protein kinases, Protéines de fusion recombinantes, Protéines végétales, Épithélium.
- pharmacologie : Nitrates.
- Nectar des plantes, Relation structure-activité.
English descriptors
- KwdEn :
- Anions (metabolism), Arabidopsis (drug effects), Arabidopsis (metabolism), Epithelium (drug effects), Epithelium (metabolism), Flowers (drug effects), Flowers (metabolism), Ion Channel Gating (drug effects), Ion Channels (metabolism), Nitrates (pharmacology), Plant Nectar (MeSH), Plant Proteins (chemistry), Plant Proteins (metabolism), Populus (drug effects), Populus (metabolism), Protein Kinases (metabolism), Recombinant Fusion Proteins (metabolism), Structure-Activity Relationship (MeSH).
- MESH :
- chemical , chemistry : Plant Proteins.
- chemical , metabolism : Anions, Ion Channels, Plant Proteins, Protein Kinases, Recombinant Fusion Proteins.
- drug effects : Arabidopsis, Epithelium, Flowers, Ion Channel Gating, Populus.
- metabolism : Arabidopsis, Epithelium, Flowers, Populus.
- chemical , pharmacology : Nitrates.
- chemical : Plant Nectar, Structure-Activity Relationship.
Abstract
Extrafloral nectaries secrete a sweet sugar cocktail that lures predator insects for protection from foraging herbivores. Apart from sugars and amino acids, the nectar contains the anions chloride and nitrate. Recent studies with Populus have identified a type of nectary covered by apical bipolar epidermal cells, reminiscent of the secretory brush border epithelium in animals. Border epithelia operate transepithelial anion transport, which is required for membrane potential and/or osmotic adjustment of the secretory cells. In search of anion transporters expressed in extrafloral nectaries, we identified PttSLAH3 (Populus tremula × Populus tremuloides SLAC1 Homologue3), an anion channel of the SLAC/SLAH family. When expressed in Xenopus oocytes, PttSLAH3 displayed the features of a voltage-dependent anion channel, permeable to both nitrate and chloride. In contrast to the Arabidopsis SLAC/SLAH family members, the poplar isoform PttSLAH3 is independent of phosphorylation activation by protein kinases. To understand the basis for the autonomous activity of the poplar SLAH3, we generated and expressed chimera between kinase-independent PttSLAH3 and kinase-dependent Arabidopsis AtSLAH3. We identified the N-terminal tail and, to a lesser extent, the C-terminal tail as responsible for PttSLAH3 kinase-(in)dependent action. This feature of PttSLAH3 may provide the secretory cell with a channel probably controlling long-term nectar secretion.
DOI: 10.1111/nph.13841
PubMed: 26831448
Affiliations:
- Allemagne
- Bade-Wurtemberg, Bavière, District de Basse-Franconie, District de Fribourg-en-Brisgau
- Fribourg-en-Brisgau, Wurtzbourg
Links toward previous steps (curation, corpus...)
Le document en format XML
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Müller</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Simon, Judy" sort="Simon, Judy" uniqKey="Simon J" first="Judy" last="Simon">Judy Simon</name><affiliation wicri:level="3"><nlm:affiliation>Chair of Tree Physiology, University of Freiburg, Freiburg, 79110, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Chair of Tree Physiology, University of Freiburg, Freiburg, 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type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Cuin, Tracey Ann" sort="Cuin, Tracey Ann" uniqKey="Cuin T" first="Tracey Ann" last="Cuin">Tracey Ann Cuin</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Fromm, Jorg" sort="Fromm, Jorg" uniqKey="Fromm J" first="Jörg" last="Fromm">Jörg Fromm</name><affiliation wicri:level="1"><nlm:affiliation>Center for Wood Sciences, University of Hamburg, Leuschnerstr. 91, Hamburg, 21031, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Wood Sciences, University of Hamburg, Leuschnerstr. 91, Hamburg, 21031</wicri:regionArea><wicri:noRegion>21031</wicri:noRegion><wicri:noRegion>21031</wicri:noRegion><wicri:noRegion>21031</wicri:noRegion></affiliation></author><author><name sortKey="Ache, Peter" sort="Ache, Peter" uniqKey="Ache P" first="Peter" last="Ache">Peter Ache</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Geiger, Dietmar" sort="Geiger, Dietmar" uniqKey="Geiger D" first="Dietmar" last="Geiger">Dietmar Geiger</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Hedrich, Rainer" sort="Hedrich, Rainer" uniqKey="Hedrich R" first="Rainer" last="Hedrich">Rainer Hedrich</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26831448</idno><idno type="pmid">26831448</idno><idno type="doi">10.1111/nph.13841</idno><idno type="wicri:Area/Main/Corpus">001943</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001943</idno><idno type="wicri:Area/Main/Curation">001943</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001943</idno><idno type="wicri:Area/Main/Exploration">001943</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">SLAH3-type anion channel expressed in poplar secretory epithelia operates in calcium kinase CPK-autonomous manner.</title><author><name sortKey="Jaborsky, Mario" sort="Jaborsky, Mario" uniqKey="Jaborsky M" first="Mario" last="Jaborsky">Mario Jaborsky</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Maierhofer, Tobias" sort="Maierhofer, Tobias" uniqKey="Maierhofer T" first="Tobias" last="Maierhofer">Tobias Maierhofer</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Olbrich, Andrea" sort="Olbrich, Andrea" uniqKey="Olbrich A" first="Andrea" last="Olbrich">Andrea Olbrich</name><affiliation wicri:level="1"><nlm:affiliation>Thünen Institute of Wood Research, Leuschnerstr. 91d, Hamburg, 21031, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Thünen Institute of Wood Research, Leuschnerstr. 91d, Hamburg, 21031</wicri:regionArea><wicri:noRegion>21031</wicri:noRegion><wicri:noRegion>21031</wicri:noRegion><wicri:noRegion>21031</wicri:noRegion></affiliation></author><author><name sortKey="Escalante Perez, Maria" sort="Escalante Perez, Maria" uniqKey="Escalante Perez M" first="María" last="Escalante-Pérez">María Escalante-Pérez</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Muller, Heike M" sort="Muller, Heike M" uniqKey="Muller H" first="Heike M" last="Müller">Heike M. Müller</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Simon, Judy" sort="Simon, Judy" uniqKey="Simon J" first="Judy" last="Simon">Judy Simon</name><affiliation wicri:level="3"><nlm:affiliation>Chair of Tree Physiology, University of Freiburg, Freiburg, 79110, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Chair of Tree Physiology, University of Freiburg, Freiburg, 79110</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Krol, Elzbieta" sort="Krol, Elzbieta" uniqKey="Krol E" first="Elzbieta" last="Krol">Elzbieta Krol</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Cuin, Tracey Ann" sort="Cuin, Tracey Ann" uniqKey="Cuin T" first="Tracey Ann" last="Cuin">Tracey Ann Cuin</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Fromm, Jorg" sort="Fromm, Jorg" uniqKey="Fromm J" first="Jörg" last="Fromm">Jörg Fromm</name><affiliation wicri:level="1"><nlm:affiliation>Center for Wood Sciences, University of Hamburg, Leuschnerstr. 91, Hamburg, 21031, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Wood Sciences, University of Hamburg, Leuschnerstr. 91, Hamburg, 21031</wicri:regionArea><wicri:noRegion>21031</wicri:noRegion><wicri:noRegion>21031</wicri:noRegion><wicri:noRegion>21031</wicri:noRegion></affiliation></author><author><name sortKey="Ache, Peter" sort="Ache, Peter" uniqKey="Ache P" first="Peter" last="Ache">Peter Ache</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Geiger, Dietmar" sort="Geiger, Dietmar" uniqKey="Geiger D" first="Dietmar" last="Geiger">Dietmar Geiger</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author><author><name sortKey="Hedrich, Rainer" sort="Hedrich, Rainer" uniqKey="Hedrich R" first="Rainer" last="Hedrich">Rainer Hedrich</name><affiliation wicri:level="3"><nlm:affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082</wicri:regionArea><placeName><region type="land" nuts="1">Bavière</region><region type="district" nuts="2">District de Basse-Franconie</region><settlement type="city">Wurtzbourg</settlement></placeName></affiliation></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anions (metabolism)</term><term>Arabidopsis (drug effects)</term><term>Arabidopsis (metabolism)</term><term>Epithelium (drug effects)</term><term>Epithelium (metabolism)</term><term>Flowers (drug effects)</term><term>Flowers (metabolism)</term><term>Ion Channel Gating (drug effects)</term><term>Ion Channels (metabolism)</term><term>Nitrates (pharmacology)</term><term>Plant Nectar (MeSH)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (metabolism)</term><term>Populus (drug effects)</term><term>Populus (metabolism)</term><term>Protein Kinases (metabolism)</term><term>Recombinant Fusion Proteins (metabolism)</term><term>Structure-Activity Relationship (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anions (métabolisme)</term><term>Arabidopsis (effets des médicaments et des substances chimiques)</term><term>Arabidopsis (métabolisme)</term><term>Canaux ioniques (métabolisme)</term><term>Fleurs (effets des médicaments et des substances chimiques)</term><term>Fleurs (métabolisme)</term><term>Nectar des plantes (MeSH)</term><term>Nitrates (pharmacologie)</term><term>Ouverture et fermeture des portes des canaux ioniques (effets des médicaments et des substances chimiques)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Populus (métabolisme)</term><term>Protein kinases (métabolisme)</term><term>Protéines de fusion recombinantes (métabolisme)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (métabolisme)</term><term>Relation structure-activité (MeSH)</term><term>Épithélium (effets des médicaments et des substances chimiques)</term><term>Épithélium (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Anions</term><term>Ion Channels</term><term>Plant Proteins</term><term>Protein Kinases</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Arabidopsis</term><term>Epithelium</term><term>Flowers</term><term>Ion Channel Gating</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Arabidopsis</term><term>Fleurs</term><term>Ouverture et fermeture des portes des canaux ioniques</term><term>Populus</term><term>Épithélium</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Epithelium</term><term>Flowers</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anions</term><term>Arabidopsis</term><term>Canaux ioniques</term><term>Fleurs</term><term>Populus</term><term>Protein kinases</term><term>Protéines de fusion recombinantes</term><term>Protéines végétales</term><term>Épithélium</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Nitrates</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Nitrates</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Plant Nectar</term><term>Structure-Activity Relationship</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Nectar des plantes</term><term>Relation structure-activité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Extrafloral nectaries secrete a sweet sugar cocktail that lures predator insects for protection from foraging herbivores. Apart from sugars and amino acids, the nectar contains the anions chloride and nitrate. Recent studies with Populus have identified a type of nectary covered by apical bipolar epidermal cells, reminiscent of the secretory brush border epithelium in animals. Border epithelia operate transepithelial anion transport, which is required for membrane potential and/or osmotic adjustment of the secretory cells. In search of anion transporters expressed in extrafloral nectaries, we identified PttSLAH3 (Populus tremula × Populus tremuloides SLAC1 Homologue3), an anion channel of the SLAC/SLAH family. When expressed in Xenopus oocytes, PttSLAH3 displayed the features of a voltage-dependent anion channel, permeable to both nitrate and chloride. In contrast to the Arabidopsis SLAC/SLAH family members, the poplar isoform PttSLAH3 is independent of phosphorylation activation by protein kinases. To understand the basis for the autonomous activity of the poplar SLAH3, we generated and expressed chimera between kinase-independent PttSLAH3 and kinase-dependent Arabidopsis AtSLAH3. We identified the N-terminal tail and, to a lesser extent, the C-terminal tail as responsible for PttSLAH3 kinase-(in)dependent action. This feature of PttSLAH3 may provide the secretory cell with a channel probably controlling long-term nectar secretion.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26831448</PMID><DateCompleted><Year>2016</Year><Month>12</Month><Day>26</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>210</Volume><Issue>3</Issue><PubDate><Year>2016</Year><Month>May</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>SLAH3-type anion channel expressed in poplar secretory epithelia operates in calcium kinase CPK-autonomous manner.</ArticleTitle><Pagination><MedlinePgn>922-33</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/nph.13841</ELocationID><Abstract><AbstractText>Extrafloral nectaries secrete a sweet sugar cocktail that lures predator insects for protection from foraging herbivores. Apart from sugars and amino acids, the nectar contains the anions chloride and nitrate. Recent studies with Populus have identified a type of nectary covered by apical bipolar epidermal cells, reminiscent of the secretory brush border epithelium in animals. Border epithelia operate transepithelial anion transport, which is required for membrane potential and/or osmotic adjustment of the secretory cells. In search of anion transporters expressed in extrafloral nectaries, we identified PttSLAH3 (Populus tremula × Populus tremuloides SLAC1 Homologue3), an anion channel of the SLAC/SLAH family. When expressed in Xenopus oocytes, PttSLAH3 displayed the features of a voltage-dependent anion channel, permeable to both nitrate and chloride. In contrast to the Arabidopsis SLAC/SLAH family members, the poplar isoform PttSLAH3 is independent of phosphorylation activation by protein kinases. To understand the basis for the autonomous activity of the poplar SLAH3, we generated and expressed chimera between kinase-independent PttSLAH3 and kinase-dependent Arabidopsis AtSLAH3. We identified the N-terminal tail and, to a lesser extent, the C-terminal tail as responsible for PttSLAH3 kinase-(in)dependent action. This feature of PttSLAH3 may provide the secretory cell with a channel probably controlling long-term nectar secretion.</AbstractText><CopyrightInformation>© 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jaborsky</LastName><ForeName>Mario</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maierhofer</LastName><ForeName>Tobias</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Olbrich</LastName><ForeName>Andrea</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Thünen Institute of Wood Research, Leuschnerstr. 91d, Hamburg, 21031, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Escalante-Pérez</LastName><ForeName>María</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Müller</LastName><ForeName>Heike M</ForeName><Initials>HM</Initials><AffiliationInfo><Affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Simon</LastName><ForeName>Judy</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Chair of Tree Physiology, University of Freiburg, Freiburg, 79110, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Krol</LastName><ForeName>Elzbieta</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cuin</LastName><ForeName>Tracey Ann</ForeName><Initials>TA</Initials><AffiliationInfo><Affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fromm</LastName><ForeName>Jörg</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Center for Wood Sciences, University of Hamburg, Leuschnerstr. 91, Hamburg, 21031, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ache</LastName><ForeName>Peter</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Geiger</LastName><ForeName>Dietmar</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hedrich</LastName><ForeName>Rainer</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>University Würzburg, Biozentrum, Julius-von-Sachs-Institut für Biowissenschaften, Julius-von-Sachs-Platz 2, Würzburg, D-97082, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>At4g04720</AccessionNumber><AccessionNumber>At4g33950</AccessionNumber><AccessionNumber>At5g24030</AccessionNumber><AccessionNumber>LN625228</AccessionNumber><AccessionNumber>LN625229</AccessionNumber><AccessionNumber>LN625230</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>02</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000838">Anions</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007473">Ion Channels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009566">Nitrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D057048">Plant Nectar</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.-</RegistryNumber><NameOfSubstance UI="D011494">Protein Kinases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000838" MajorTopicYN="N">Anions</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004848" MajorTopicYN="N">Epithelium</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D035264" MajorTopicYN="N">Flowers</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015640" MajorTopicYN="N">Ion Channel Gating</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007473" MajorTopicYN="N">Ion Channels</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009566" MajorTopicYN="N">Nitrates</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057048" MajorTopicYN="N">Plant Nectar</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011494" MajorTopicYN="N">Protein Kinases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013329" MajorTopicYN="N">Structure-Activity Relationship</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">S-type anion channels</Keyword><Keyword MajorTopicYN="N">SLAC/SLAH</Keyword><Keyword MajorTopicYN="N">anion channels</Keyword><Keyword MajorTopicYN="N">extrafloral nectaries</Keyword><Keyword MajorTopicYN="N">poplar</Keyword><Keyword MajorTopicYN="N">transepithelial transport</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>10</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>12</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>2</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>2</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26831448</ArticleId><ArticleId IdType="doi">10.1111/nph.13841</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>Bavière</li><li>District de Basse-Franconie</li><li>District de Fribourg-en-Brisgau</li></region><settlement><li>Fribourg-en-Brisgau</li><li>Wurtzbourg</li></settlement></list><tree><country name="Allemagne"><region name="Bavière"><name sortKey="Jaborsky, Mario" sort="Jaborsky, Mario" uniqKey="Jaborsky M" first="Mario" last="Jaborsky">Mario Jaborsky</name></region><name sortKey="Ache, Peter" sort="Ache, Peter" uniqKey="Ache P" first="Peter" last="Ache">Peter Ache</name><name sortKey="Cuin, Tracey Ann" sort="Cuin, Tracey Ann" uniqKey="Cuin T" first="Tracey Ann" last="Cuin">Tracey Ann Cuin</name><name sortKey="Escalante Perez, Maria" sort="Escalante Perez, Maria" uniqKey="Escalante Perez M" first="María" last="Escalante-Pérez">María Escalante-Pérez</name><name sortKey="Fromm, Jorg" sort="Fromm, Jorg" uniqKey="Fromm J" first="Jörg" last="Fromm">Jörg Fromm</name><name sortKey="Geiger, Dietmar" sort="Geiger, Dietmar" uniqKey="Geiger D" first="Dietmar" last="Geiger">Dietmar Geiger</name><name sortKey="Hedrich, Rainer" sort="Hedrich, Rainer" uniqKey="Hedrich R" first="Rainer" last="Hedrich">Rainer Hedrich</name><name sortKey="Krol, Elzbieta" sort="Krol, Elzbieta" uniqKey="Krol E" first="Elzbieta" last="Krol">Elzbieta Krol</name><name sortKey="Maierhofer, Tobias" sort="Maierhofer, Tobias" uniqKey="Maierhofer T" first="Tobias" last="Maierhofer">Tobias Maierhofer</name><name sortKey="Muller, Heike M" sort="Muller, Heike M" uniqKey="Muller H" first="Heike M" last="Müller">Heike M. Müller</name><name sortKey="Olbrich, Andrea" sort="Olbrich, Andrea" uniqKey="Olbrich A" first="Andrea" last="Olbrich">Andrea Olbrich</name><name sortKey="Simon, Judy" sort="Simon, Judy" uniqKey="Simon J" first="Judy" last="Simon">Judy Simon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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